Collect

Blood in 4mL DkGrn NaHep  (2 tubes needed). Collect only Mon-Fri. On Fridays, must be received to Burnet lab by 1300.

Minimum Collection Volume

Contact Lab for minimum volumes (based on ALC)

Shipping/Handling Instructions (Lab Use Only)

Do not spin. Critical: Room Temp

External Client Shipping and Handling

Room temperature only.  Must be received in lab within 24 hours of collection. 

Stability (from collection to initiation)

Room Temp 24 Hours 

Performing Lab

CBDI DIL:
Phone: 513-636-4685
Fax: 513-636-3861
Email: CBDILabs@cchmc.org

Synonyms

  • Cytotoxic T Lymphocyte
  • CTL

Tests Included

CTL function

Methodology

Chromium release

Reported

14 days

Interpretive Data

Cytotoxic T lymphocytes (CTL) are a subset of lymphocytes that have the ability to lyse target cells bearing specific antigens. To assess the functionality of CTLs, target cells previously primed to contain radioactive chromium within the cell membrane are incubated with patient or control lymphocytes.

Lysis of the target cells by the CTLs is measured by the amount of chromium released into the supernatant.

CPT Codes

86352
Collection

Collect

Blood in 4mL DkGrn NaHep  (2 tubes needed). Collect only Mon-Fri. On Fridays, must be received to Burnet lab by 1300.

Minimum Collection Volume

Contact Lab for minimum volumes (based on ALC)

Shipping/Handling Instructions (Lab Use Only)

Do not spin. Critical: Room Temp

External Client Shipping and Handling

Room temperature only.  Must be received in lab within 24 hours of collection. 

Stability (from collection to initiation)

Room Temp 24 Hours 

Performing Lab

CBDI DIL:
Phone: 513-636-4685
Fax: 513-636-3861
Email: CBDILabs@cchmc.org
Ordering

Synonyms

  • Cytotoxic T Lymphocyte
  • CTL

Tests Included

CTL function

Methodology

Chromium release

Reported

14 days
Result Interpretation

Interpretive Data

Cytotoxic T lymphocytes (CTL) are a subset of lymphocytes that have the ability to lyse target cells bearing specific antigens. To assess the functionality of CTLs, target cells previously primed to contain radioactive chromium within the cell membrane are incubated with patient or control lymphocytes.

Lysis of the target cells by the CTLs is measured by the amount of chromium released into the supernatant.
Laboratory Personnel Use

CPT Codes

86352